The correlation of HBeAg expression and HBV-DNA in serum or peripheral blood mononuclear cells in patients with chronic hepatitis B.
- Author:
Yu-Qiang MI
1
;
Shu-Ren LIANG
;
Hong ZHANG
;
Shu-Wen ZHENG
;
Shun-Tian LI
;
Wu-Kui CAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Antibodies, Viral; blood; DNA, Viral; blood; genetics; Enzyme-Linked Immunosorbent Assay; Female; Hepatitis B; genetics; immunology; Hepatitis B e Antigens; immunology; Hepatitis B, Chronic; blood; virology; Humans; Leukocytes, Mononuclear; virology; Male; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; methods; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(3):261-263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between HBeAg expression and HBV-DNA in serum and peripheral blood mononuclear cells (PBMCs).
METHODS208 patients with chronic hepatitis B were included in this present study. HBV-DNA in the PBMCs were performed by polymerase chain reaction (PCR), with the serum HBV-DNA level being determined by the way of fluoresces quantities PCR (FQ-PCR). Meanwhile, HBV-GM was also detected via enzyme-linked immunosorbent assay (ELISA).
RESULTSThere were 106 patients for positivity in the HBV-DNA level of PBMCs with 102 for negativity, in which the HBV-DNA high levels (HBV DNA load > or = 1.0E5) in serum were 91.5%, 45.1% (chi2=52.12, P>0.01) respectively, with 76.4% and 50.9% (chi2=21.55, P>0.01) for the positive percentage of HBeAg expression.
CONCLUSIONA significantly positive correlation was found between HBV-DNA in PBMCs and serum HBV-DNA along with the positive percentage of HBeAg, indicating that obvious PBMCs' increase infected by HBV in patients with positivity of HBeAg and high level of serum HBV-DNA.
