Effect of cytomegalovirus infection on expression of matrix metalloproteinase-2 in human endothelial cells.
- Author:
Rui-Jin WANG
1
;
Hou-Zhen TUO
;
De-Xin WANG
;
Jia-Wei WANG
;
Zi-Jing FENG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Cytomegalovirus; physiology; Endothelial Cells; cytology; metabolism; virology; Gene Expression Regulation, Enzymologic; Host-Pathogen Interactions; Humans; Matrix Metalloproteinase 2; biosynthesis; genetics; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Umbilical Veins; cytology
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(3):264-266
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of cytomegalovirus infection on expression of matrix metalloproteinase-2 in human endothelial cells.
METHODSHuman umbilical vein endothelial cells were cultured in vitro. Cells between 3-6 passages were infected with cytomegalovirus for different time. Expression of matrix metalloproteinase-2 mRNA was analyzed by reverse transcription-polymerase chain reaction, matrix metalloproteinase-2 activity was detected by gel zymography.
RESULTSExpression of matrix metalloproteinase-2 mRNA and its activity 6 hours after infection was almost equal to control, and was greatly enhanced 12 and 24 hours after infection.
CONCLUSIONCytomegalovirus infection up-regulates expression of matrix metalloproteinase-2 in human endothelial cells. It might be one of the mechanisms that cytomegalovirus is involved in atherosclerosis.
