Cyclosporine A improves the nuclear entry of hepatitis B virus core protein in HepG2.2.15 cells.
- Author:
Xiao-ben PAN
1
,
2
;
Jin-chao HAN
;
Yan GAO
;
Lai WEI
Author Information
- Publication Type:Journal Article
- MeSH: Active Transport, Cell Nucleus; Apoptosis; Cell Line, Tumor; Cell Nucleus; metabolism; Cyclosporine; pharmacology; Cytoplasm; metabolism; Hepatitis B Core Antigens; analysis; metabolism; Hepatitis B Surface Antigens; analysis; Humans; In Situ Nick-End Labeling; Phosphorylation
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(4):310-312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe present aimed to observe the effect of phosphatase inhibitor cyclosporine A on the subcellular location and on expression of HBcAg in HepG2.2.15 cells.
METHODSThirty micrograms/ml of cyclosporine A (CSA) was added into HepG2.2.15 cell culture system and on days 2 and 4 HBcAg and HBsAg were respectively stained with fluorescent immunocytochemistry and observed under confocal microscope. Cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling method.
RESULTSHBcAg was mostly expressed in cytoplasm in the control HepG2.2.15 cells. After 2 days CSA administration of the expression of HBcAg and HBsAg in cytoplasm significantly decreased and the signals of HBcAg in nucleus increased , whereas the HBcAg was still mainly expressed in nucleus in about 1/4 of the cells. Cell apoptosis was observed in about 30% of the cells.
CONCLUSIONCSA improves the nuclear entry of core protein. The increase of HBcAg in nucleus was likely to be related with it's phosphorylation and cell aging or apoptosis.
