Development and evaluation of a double antigen sandwich ELISA for the detection of total antibodies against hemorrhagic fever with renal syndrome virus.
- Author:
Quan-fu ZHANG
1
;
Jian-dong LI
;
Wei-hong LI
;
Chuan LI
;
Qin-zhi LIU
;
Mi-fang LIANG
;
De-xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; blood; Enzyme-Linked Immunosorbent Assay; methods; Hantaan virus; immunology; Humans; Reproducibility of Results; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(4):386-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo develop and evaluate a method for detection of the total antibodies against hemorrhagic fever with renal syndrome (HFRS) virus with improved sensitivity and simplified operation procedure.
METHODSThe nucleic proteins of hantavirus were used as coating antigens as well as detection antigens labeled with horse radish peroxidase (HRP). The operation protocol was established, optimized and compared with indirect fluorescence assay (IFA).
RESULTSThe specificity of this method was 100 percent in the test of different human sera and 4-8 times more sensitive than IFA. And, it is simpler without requiring any change of the reagents, different sources of samples did not affect the results of the test.
CONCLUSIONThis method is specific, sensitive and simple for detection of the total antibodies in sera against hantavirus, could be used for the screening of Hantavirus infection in human and host rodent animals.
