Evaluation on the use of detection of hepatitis C core antigen for screening blood donor.
- Author:
Fu-cheng WANG
1
;
Zhi-yong SHI
;
Jun CAI
;
Jun SU
Author Information
- Publication Type:Journal Article
- MeSH: Blood Donors; Enzyme-Linked Immunosorbent Assay; Hepatitis C Antibodies; blood; Hepatitis C Antigens; blood; Humans; RNA, Viral; blood; Reverse Transcriptase Polymerase Chain Reaction; Viral Core Proteins; blood
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(4):389-390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the value of detection of hepatitis C virus core antigen (HCV-cAg) for screening blood donor by using the internal reagent enzyme immunoassay (EIA) and anti-HCV antibody.
METHODSThe first and repeat assays were performed for detection of serum anti-HCV and HCV-cAg ELISA in 3972 donor's serum specimens from August to October of 2004. Twenty-five donors positive for anti-HCV were tested with HCV-cAg EIA kits and the results were compared with the results of HCV RNA determination with RT-PCR method.
RESULTSIn 3972 donor's serum samples, only 1 serum specimen was positive for HCV RNA identification among 10 specimens which were positive for anti-HCV in first assays, and only 1 serum specimens was positive for HCV RNA identification among 12 specimens positive for anti-HCV in repeat assays, only 2 serum specimens were positive HCV RNA identification in 3 specimens which were positive for HCV-cAg assays.
CONCLUSIONThe sensitivity of HCV-cAg ELISA is similar to HCV RT-PCR, but it is much cheaper. Therefore, HCV-cAg ELISA and anti-HCV may be used together to screen blood donor.
