Molecular characterization of hantavirus Zhejiang isolate ZT10 strain from M. fartis.
- Author:
Jing-qing WENG
1
;
Rong-hui XIE
;
Ping-ping YAO
;
Ming-hong LI
;
Fang XU
;
Yi-yu LU
;
Han-ping ZHU
;
Qing-rong CUI
;
Z Y ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Viral; analysis; Cell Line; Cloning, Molecular; DNA, Complementary; genetics; DNA, Viral; genetics; Fluorescent Antibody Technique, Direct; Hantavirus; classification; genetics; isolation & purification; Phylogeny; Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(1):2-5
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo learn about the complete genomic sequence of the Seoul virus strain ZT10 isolated from M. fartis.
METHODSThe total RNA was extracted from the infected Vero E6 cells and amplified by RT-PCR. The purified PCR products were cloned into T-vector and sequenced.
RESULTSThe results demonstrated that the complete genome of ZT10 was comprised of L(6530), M(3651) and S(1753) segments which encoded 2151-1133 and 429 amino acids respectively.
CONCLUSIONAnalysis of sequence revealed that the ZT10 belonged to Seoul virus. The nucleotide sequence identity of the M gene with Seoul virus was 84.0%-96.3%. The identity with Hantan vrisu (Prospect Hill virus, Tula virus) isolated from M. fartis was 57.5%-60.9%. The sequence identity of the S gene with Seoul virus was 87.9%-96.0% at nucleotide level and 96.9%-97.9% at amino acid level.
