Development and application of a two-step MacELISA for the early diagnosis of hemorrhagic fever with renal syndrome.
- Author:
Quan-fu ZHANG
1
;
Jian-dong LI
;
Wei-hong LI
;
Chuan LI
;
Qin-zhi LIU
;
Mi-fang LIANG
;
De-xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Case-Control Studies; Enzyme-Linked Immunosorbent Assay; methods; Hemorrhagic Fever with Renal Syndrome; blood; diagnosis; Humans; Immunoglobulin M; blood; Reproducibility of Results; Sensitivity and Specificity; Time Factors
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(1):6-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure.
METHODSThe nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA.
RESULTSThis method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from human patient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%.
CONCLUSIONThis method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.
