Cloning and sequence analysis of envelope glycoprotein G2 gene of hantavirus in Shandong province.
- Author:
Shao-xia SONG
1
;
Zhi-yu WANG
;
Zhen-qiang BI
;
Zhi-qiang WANG
;
Ze-xin TAO
;
Yu-lu WANG
;
Yan-yan SONG
;
Gui-ting WANG
;
Hong-zhi XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; China; Cloning, Molecular; Hantavirus; genetics; Mice; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Viral Envelope Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2008;22(1):9-11
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the cloning vector of glycoprotein G2 gene of hantavirus (HV), to analyze the sequence of G2 gene by the phylogenetic tree, and to study the differences among glycoprotein G2 genes from the world around.
METHODSEnvelope glycoprotein G2 gene was amplified from four specimens of Shandong province by RT-PCR, and the product recombined into the PMD-18T vector. The clones that carry the G2 gene were identified. After sequencing, the gene sequence was handled with the software DNASTAR, compared with 24 strains worldwide and the phylogenetic tree was drawn.
RESULTSHV G2 gene was amplified by RT-PCR from 4 specimens, named GM04-38.G2, ZB8.G2, JUN5-14.G2, RCH5.G2, respectively. The map of the phylogenetic tree showed that all the 4 strains belonged to SEO-type hantavirus. The analysis of the sequence showed that all the four HV strains had the highest rates of homology with Z37 strain. The sequence homology of SEO-type HV strains was from 82.3% to 99.8%.
CONCLUSIONThe four cloning vectors containing the glycoprotein G2 genes were successfully constructed. Envelope glycoprotein G2 gene of four specimens from Shandong province had high homology rates.
