Epidemiologic and etiologic characteristics of diarrheagenic Escherichia coli infection in population in Shenzhen.

Yinghui LI; Yaqun Qiu; Huixia Xian; Xiaolu Shi; Yiman Lin; Qiongcheng Chen; Min Jiang; Yixiang Jiang; Lulu HU; Qinghua HU

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Epidemiologic and etiologic characteristics of diarrheagenic Escherichia coli infection in population in Shenzhen.

VernacularTitle:深圳市腹泻人群致泻性大肠埃希菌流行及病原特征研究
Author: Yinghui LI ¹ ; Yaqun QIU ¹ ; Huixia XIAN ¹ ; Xiaolu SHI ¹ ; Yiman LIN ¹ ; Qiongcheng CHEN ¹ ; Min JIANG ¹ ; Yixiang JIANG ¹ ; Lulu HU ² ; Qinghua HU ³ ;
Author Information

1. Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
2. School of Life Sciences, Shenzhen University, Shenzhen 518060, China.
3. Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
Publication Type:Journal Article
MeSH: Adult; Child, Preschool; China; epidemiology; Diarrhea; epidemiology; microbiology; Disease Outbreaks; Electrophoresis, Gel, Pulsed-Field; Enterotoxigenic Escherichia coli; classification; isolation & purification; Escherichia coli Infections; epidemiology; Humans; Real-Time Polymerase Chain Reaction; Serotyping; Young Adult
From: Chinese Journal of Epidemiology 2016;37(1):115-118
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo understand the epidemiologic and etiologic characteristics of diarrheagenic Escherichia (E.) coli infections in Shenzhen.
METHODSStool samples were collected from acute diarrheal patients in four sentinel hospitals in Shenzhen and diarrheagenic E. coli strains were isolated and identified with multiplex real-time PCR. Serotyping and pulsed-field gel electrophoresis (PFGE) typing were conducted for the diarrheagenic E. coli isolates.
RESULTSA total of 74 diarrheagenic E. coli strains were isolated from 1 823 stool samples (4.06%). The patients were mainly young children aged <3 years and adults aged 20-39 years, and the infections mainly occurred during May-September of a year. Enterotoxigenic E. coli (ETEC) and enteropathognic E. coli (EPEC) were predominant (45.9% and 31.1%). Serogroups and PFGE patterns varied among the diarrheagenic E. coli isolates. However, serogroup O159 were predominant in ETEC and there were 5 clusters with ≥2 strains sharing same PFGE patterns.
CONCLUSIONSETEC and EPEC were predominant in diarrheagenic E. coli strains isolated from diarrheal patients in Shenzhen. Age and season specific characteristics of diarrheagenic E. coli infections were observed. The serotypes and PFGE patterns of diarrheagenic E. coli strains varied. Close attention should be paid to the possible ETEC outbreak.