Study on VNTR diversity of clinical Mycobacterium tuberculosis isolates from Qinghai.

Bin LI; Haican Liu; Zhaofen Wang; Email: Kristy538@163com; Yongcheng MA; Xiaodong SU; Mingxia Jiang; Kanglin Wan; Shou Liu; Xiuqin Zhao; Shugen QU

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Study on VNTR diversity of clinical Mycobacterium tuberculosis isolates from Qinghai.

Author: Bin LI ¹ ; Haican LIU ² ; Zhaofen WANG ³ ; Email: KRISTY538@163.COM. ; Yongcheng MA ⁴ ; Xiaodong SU ¹ ; Mingxia JIANG ⁴ ; Kanglin WAN ² ; Shou LIU ¹ ; Xiuqin ZHAO ² ; Shugen QU ¹
Author Information

1. Medical School of Qinghai University, Xining 810001, China.
2. State Key Laboratory for Communicable Disease Prevention and Control, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
3. Medical School of Qinghai University, Xining 810001, China
4. Institute for Communicable Disease Control and Prevention, Qinghai Provincial Center for Disease Control and Prevention.
Publication Type:Journal Article
MeSH: China; DNA, Bacterial; genetics; Genetic Variation; Genotype; Humans; Minisatellite Repeats; Mycobacterium tuberculosis; genetics; Polymerase Chain Reaction; Polymorphism, Genetic; Sequence Analysis, DNA; Tuberculosis; microbiology
From: Chinese Journal of Epidemiology 2015;36(10):1158-1161
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the variable number tandem repeats (VNTR) genetic polymorphisms, genotyping and distribution pattern of clinical Mycobacterium (M.) tuberculosis isolates from Qinghai province.
METHODSThe clinical M. tuberculosis strains isolated from the patients with tuberculosis and related background data were collected from Qinghai Provincial Center for Disease Control and Prevention from 2009 to 2012. Genotyping was conducted by using multiple locus VNTR analysis (MLVA). Genomic DNA was extracted and 15 VNTR loci were amplified with PCR and the PCR products were detected with gel electrophoresis. The VNTR diversity and clusters of genotyping were analyzed with BioNumerics (Version 5.0).
RESULTSA total of 251 clinical M. tuberculosis isolates were analyzed with 15 VNTR loci showing that there were great genetic diversity in these isolates. Six of the 15 VNTR loci, showed that the Hunter-Gaston index (HGI) were higher than 0.6, in which the highest resolution was MIRU26. The clusters of genotyping showed that these isolates could be categorized into four gene clusters and 238 genotypes. The four gene clusters accounted for 4.9%, 91.9%, 1.6% and 1.6% of the clinical isolates, respectively.
CONCLUSIONThe results showed that there is great variety of VNTR genetic polymorphisms in clinical M. tuberculosis isolates in Qinghai province.