Study on virulence factors of Candida tropicalis isolated from clinical samples
10.3760/cma.j.issn.0254-6450.2015.10.027
- VernacularTitle:热带假丝酵母菌临床分离株毒力表型特征研究
- Author:
Shuanbao YU
1
;
Wenge LI
;
Jie CHE
;
Funing BIAN
;
Jinxing LU
;
Yuan WU
Author Information
1. 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室感染性疾病诊治协同中心
- Keywords:
Candida tropicalis;
Virulence factors;
Aspartyl proteinases;
Phospholipases;
Hemolytic activity
- From:
Chinese Journal of Epidemiology
2015;36(10):1162-1166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the in vitro production of virulence factors for Candida (C.) tropicalis,including aspartyl proteinases,phospholipases and hemolytic activities,describe the regulation of virulence factors varying with time in C.tropicalis,and analyze the differences in aspartyl proteinases and hemolytic activities of C.tropicalis isolated from anatomically distinct sites.Methods A total of 64 C.tropicalis strains were spot-inoculated onto bovine albumin agar,egg yolk agar and sheep blood agar plates,respectively.Then the plates were incubated for 24,48 and 72 hour at 37 ℃,respectively.The aspartyl proteinases,phospholipase and hemolytic activities were determined at each time point,respectively.Results All the C.tropiclais isolates showed positive aspartyl proteinases and hemolytic activities at each time point,but no phospholipases activity was detected in C.tropicalis.On comparison of aspartyl proteinases and hemolytic activities at different time points,aspartyl proteinases activity at 48 and 72 hour was higher than that at 24 hour.During 72 hour,hemolytic activity of C.tropicalis increased.No statistical significant differences in aspartyl proteinases and hemolytic activities of C.tropicalis were observed among different infection sites (P=0.368 and 0.985).Conclusion The C.tropicalis clinical isolates in China have aspartyl proteinases activity,hemolytic activity,but have no phospholipase activity.
