Etiologic characteristics of food-borne Vibrio parahaemolyticus strains isolated in Guangdong,2014
10.3760/cma.j.issn.0254-6450.2015.11.020
- VernacularTitle:广东省2014年食源性副溶血弧菌病原学特征分析
- Author:
Baisheng LI
1
;
Hailing TAN
;
Bixia KE
;
Dongmei HE
;
Changwen KE
;
Yonghui ZHANG
Author Information
1. 广东省疾病预防控制中心病原微生物检验所
- Keywords:
Vibrio parahaemolyticus;
Etiologic characteristic;
Pulsed field gel electrophoresis;
Multi locus sequence typing
- From:
Chinese Journal of Epidemiology
2015;36(11):1283-1287
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the serotypes, antimicrobial resistance, virulence genes and molecular characteristics of food-borne Vibrio parahaemolytieus isolated in Guangdong province in 2014.Methods Sixty V.parahaemolyticus strains were tested in this study.The serotyping and antibiotic resistance test were conducted, while the tdh and trh gens of the strains were detected with conventional PCR, and pulsed field gel electrophoresis (PFGE) and multiple locus sequence typing (MLST) were conducted too.Results The 60 strains belonged to 13 serotypes, the major serotypes included O3 : K6, O4: K8, O 1 : K36 and O4: KUT.The antibiotic resistance test indicated the isolates were highly resistant to ampicillin (100.0％), sulfonamides (43.3％) and cefalotin (28.3％).Up to 56.7％(34/60) of the strains were resistant to two or more antibiotics, and 2 strains showed resistance to three antibiotics.The virulence gene detection indicated that 63.3％ (38/60) of the strains carded tdh+trh-, while only 1 strain carried tdh+trh+.The 60 strains digested by Not I belonged to 48 different PFGE patterns and 3 clusters.The cluster B included the strains isolated from sporadic food borne cases with serotype of O3:K6 and similarity of 62.6％-100.0％.The cluster C included O4:K8 strains with the PFGE pattem similarity of 56.7％-62.5％.The MLST indicated that the 60 strains had 26 sequence types (STs).The ST-3 was predominant, including 33 strains (serotypes O3:K6 and O1 : K36).The four O4:K8 strains formed another predominant colony, which was different from ST-3.Conclusion The etiologic characteristics of V.parahaemolyticus varied, which might be one of the reasons for high incidence of food-bome V.parahaemolyticus infection in Guangdong.The molecular characteristics of O4: K8 strain were differem from the other predominant serotypes.Close attention should be paid to the possible outbreak caused by O4: K8 strain in this area.
