Genetic diversity of Francisella tularensis subsp.holarctica in China
10.3760/cma.j.issn.0254-6450.2015.12.021
- VernacularTitle:中国土拉弗朗西斯菌holarctica亚种的遗传多样性
- Author:
Yanhua WANG
1
;
Yao PENG
;
Lianxu XIA
Author Information
1. 中国疾病预防控制中心传染病预防控制所
- Keywords:
Francisella tularensis;
Tularemia;
Phylogeography;
Single-nucleotide polymorphism;
Insertion-deletion;
Multiple-locus variable number tandem repeat analysis
- From:
Chinese Journal of Epidemiology
2015;36(12):1410-1414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the genetic relationship between the Chinese and the foreign species of Francisella tularensis.Methods Based on our own findings and from the literature,17 SNP,4 INDEL,and 12 VNTR were selected for phylogenetic analysis on 39 strains of F.tularensis,including 10 strains of Chinese F.tularensis and 29 strains of foreign F.tularensis that had been sequenced and published.SNP-INDEL and MLVA were used for the separation and combination.Results Data from the combined analysis indicated that 3 strains of Chinese F.tularensis with Japanese FSC022 were assigned to B5;3 strains,with Swedish FSC200 to B1;3 strains with American OSU18 to B2 and 1 strain with French FTNF002-00,German F92,and American OR96246 to B4,respectively.10 strains of Chinese F.tularensis were assigned to 4 clades and the result demonstrated a wide diversity of F.tularensis subsp.holarctica in China.Conclusion A set of simple and robust typing tools for F.tularensis subsp.holarctica were established in this study.Based on the results,F.tularensis subsp.holarctica might have had its origins in Asia.
