Inhibitory Effects of miR-146b-5p on Cell Migration and Invasion of Pancreatic Cancer by Targeting MMP16
10.1007/sl1596-011-0481-5
- Author:
LIN FAN
1
;
WANG XIN
;
JIE ZHIGANG
;
HONG XIAOQUAN
;
LI XU
;
WANG MIN
;
YU YAHONG
Author Information
1. Department of Biliopancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China
- Keywords:
miR-146b-5p;
pancreatic cancer;
matrix metalloproteinase;
migration;
invasion
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2011;31(4):509-514
- CountryChina
- Language:Chinese
-
Abstract:
Previous studies have shown that miRNAs participate in a wide range of biological functions and play important roles in various human diseases including cancer.We found miR-146b-5p significantly dysregulated in human pancreatic cancer cells by qRT-PCR.To demonstrate its function and regulation mechanism,we overexpressed miR-146-5p by transfecting the mimics.Our data showed that miR-146b-5p overexpression significantly reduced the abilities of migration and invasion of MIA PaCa-2 pancreatic cancer cells.Furthermore,we found that matrix metalloproteinase 16 (MMP16) was a downstream target of miR-146b-5p by dual-luciferase reporter assay.Altogether,our findings suggest that miR-146b-5p may be involved in pancreatic cancer cell migration and invasion by targeting MMP16,and miR-146b-5p may be a potential therapeutic target for the pancreatic cancer.
