The Protective Effect of EGB761 on Vessels of Denervated Gastrocnemius in Rats and Its Mechanism
10.1007/sl1596-011-0678-7
- Author:
ZHANG DONGYI
1
,
2
;
WU RUI
;
KANG HAO
;
HONG GUANGXIANG
;
KANG SHENSONG
;
ZHANG ZHENGWEN
Author Information
1. Chinese PLA Postgraduate Medical School Beijing 100853, China
2. Department of Plastic Surgery, Henan Provincial People's Hospital, Zhengzhou 450000,China
- Keywords:
EGB761;
denervation;
muscular atrophy;
vascular degradation
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2011;31(6):789-793
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the protective effect of EGB761 on blood vessels of denervated gastrocnemius of rat and its possible mechanism.Fifteen male adult SD rats were randomly divided into three groups:normal control group (n=3),control group (n=6) and EGB761-treated group (n=6).The rats in the control and EGB761-treated group underwent a neurotomy to bilateral sciatic nerves.Then,they were administered EGB761 [100 mg/(kg·d)] and isovolumic normal saline,respectively by gavage everyday.No treatment was given to the rats in the normal control group.Gastrocnemius was harvested at 1 and 3 week(s) postoperatively in each group.Immunohistochemical method was used to detect the ratio of capillary/fiber (CFR) of denervated gastrocnemius and the expression of VEGF,fetal liver kinase -l(Flk-1) receptor and HSP70 in the vascular wall.The results showed that in the normal control group,VEGF,Flk-1 and HSP70 were expressed in the vessel wall of gastrocnemius,with Flk-1 expressed only in the endothelial cell of vessels.CFR in the EGB761-treated group was significantly higher than that in the control group at 1 week and 3 week(s) after neurotomy.The expression of VEGF and Flk-1 in the vessel wall of both control and EGB761-treated group was much lower than that in the normal control group,and the expression of these proteins in the EGB761-treated group was decreased as compared with that in the control group.The expression of HSP70 in the vessel wall of both control and EGB761-treated groups was enhanced when compared with that in the normal control group,and it was substantially augmented in the EGB761-treated group in comparison to the control group.It was concluded that EGB761 has a protective effect on blood vessels of denervated gastrocnemius,which is related to the increased HSP70 expression but not the expression of VEGF and its receptor Flk-1.
