Osteogenic Effect of Low Intensity Pulsed Ultrasound on Rat Adipose-derived Stem Cells in vitro
10.1007/sl1596-012-0013-y
- Author:
JIANG TING
1
,
2
;
XU TAO
;
GUO FENGJING
;
CHEN ANMIN
;
XIAO ZHENGZHENG
;
ZHANG DI
Author Information
1. Department of Orthopedics,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China
2. Department of Orthopedics, the Third Affiliated Hospital, Anhui Medical Univeristy, Hefei 230031, China
- Keywords:
low intensity pulsed ultrasound;
adipose-derived stem cells;
osteogenic differentiation
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2012;32(1):75-81
- CountryChina
- Language:Chinese
-
Abstract:
The osteogenic in vitro effect of low intensity pulsed ultrasound (LIPUS) on SD rat adipose-derived stem cells (ADSCs) was investigated.Rat ADSCs underwent LIPUS (intensity=100 mW/cm2) or sham exposure for 8 min per treatment once everyday in vitro,and then the alkaline phosphatase (ALP) activity and mineralized nodule formation were assessed to evaluate the osteogenic effect of LIPUS on ADSCs.To further explore the underlying mechanism,the osteogenic-related gene mRNA expression was determined by using reverse transcriptase-polymerase chain reaction (RT-PCR) at 1st,3rd,5th,7th day after exposure repectively.Westen blot was used to evaluate the protein expression levels of two osteogenic differentiation associated genes at 7th and 14th day repectively.It was found that ALP activity was increased after LIPUS exposure and LIPUS resulted in mineralized nodule formation of ADSCs in vitro.LIPUS-treated ADSCs displayed higher mRNA expression levels of runt-related transcription factor 2 (Runx2),osteocalcin (OCN),ALP and bone sialoprotein (BSP) genes than controls,and the protein levels of Runx2 and BSP were also increased.The results suggested that LIPUS may induce the osteogenic differentiation of ADSCs in vitro.
