Correlation between Physical Status of Human Papilloma Virus and Cervical Carcinogenesis
10.1007/sl1596-012-0017-7
- Author:
LI KEZHEN
1
;
JIN XIN
;
FANG YONG
;
WANG CHANGYU
;
GONG MEI
;
CHEN PINGBO
;
LIU JIA
;
DENG DONGRUI
;
AI JIHUI
Author Information
1. Cancer Biology Research Center, Tongji Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
- Keywords:
human papilloma virus;
cervical cancer;
genotyping;
physical status;
integration
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2012;32(1):97-102
- CountryChina
- Language:Chinese
-
Abstract:
The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervical carcinogenesis were investigated.HPV genotyping was performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from 198 women.Multiple PCR was done to detect physical status of HPV-1 6 in a series of cervical liquid-based cytology samples and biopsy specimens obtained from different cervical lesions with HPV-16 infection,including 112 specimens with cervical cancer,151 specimens with CIN Ⅰ,246 specimens with CIN Ⅱ and 120 specimens with CINⅢ.The results showed that there were 112 cervical cancer samples (56.57％ of total cervical cancer patients) with HPV-16 infection.The frequency of HPV-16 pure integration was 65.18％ (73/112),56.57％ (47/120),23.58％ (58/246) and 7.95％ (12/151) in cervical cancer,CINⅢ,CIN Ⅱ and CIN Ⅰ patients respectively.In situ hybridization was performed on some paraffin-embedded sections of CIN Ⅱ,CINⅢ and cervical cancer to verify the physical status of HPV-16 infection.Significant difference was observed between cervical cancer and CIN Ⅰ,CIN Ⅱ,CINⅢ in the frequency of HPV-16 integration (P＜0.01).It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer.In the case of HPV-16 infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration.The application of testing HPV genotyping and physical status based on detection ofHC- Ⅱ HPV DNA would be in favor of predicting the prognosis of cervical precancerosis and enhancing the screening accuracy of cervical cancer.
