Cloning of human brain-derived neurotrophin-6 gene and its expression in procaryotic cell.
- Author:
Chengwu ZHANG
1
;
Qingsong CAI
;
Xu ZHUANG
;
Chaoyang ZHAI
;
Yu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Brain; metabolism; Cloning, Molecular; DNA, Complementary; genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli; genetics; Gene Expression; Molecular Sequence Data; Nerve Growth Factors; chemistry; genetics; metabolism; Plasmids; genetics; Protein Structure, Tertiary; RNA; genetics; isolation & purification; Recombinant Proteins; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid
- From: Chinese Journal of Medical Genetics 2002;19(6):475-478
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone human brain-derived neurotrophin-6(NT-6) gene and to observe its expression in the procaryotic cell.
METHODSTotal RNA was extracted from aborted antenatal cerebral cortex, and cDNA fragment of NT-6 was amplified through reverse transcript-polymerase chain reaction. After being incised and recovered, the NT-6 gene was cloned into pBK-CMV plasmid to construct a NT-6 gene expression vector. Expression of NT-6 gene in Escherichia coli was studied after being induced by isopropyl beta-D-thiogalactoside(IPTG).
RESULTSThe NT-6 gene expression vector was constructed and Escherichia coli with recombinant vector expressed specific protein after induction by IPTG.
CONCLUSIONThe cloning of human brain-derived NT-6 gene provides a basis for further studying the structure, function and clinical application of NT-6.
