Cloning and tissue expression analysis of up-regulated cDNA fragment in human gastric cancer.
- Author:
Hong LI
1
;
Meng-wei WANG
;
Yong SHAO
;
Gang-shi WANG
;
Wei-di YOU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Northern; Cloning, Molecular; DNA, Complementary; chemistry; genetics; Female; Gene Expression Regulation, Neoplastic; Genetic Predisposition to Disease; genetics; HL-60 Cells; HeLa Cells; Humans; K562 Cells; Molecular Sequence Data; Sequence Analysis, DNA; Stomach Neoplasms; genetics; Tumor Cells, Cultured
- From: Chinese Journal of Medical Genetics 2003;20(1):12-14
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify novel human gastric cancer-associated susceptibility gene for early diagnosis and treatment of gastric cancer.
METHODSA primer was designed for 3'-rapid amplification of cDNA end(RACE) and amplified fragments were cloned, then they were analyzed by sequencing. Compared with ESTs in Genbank, the EST fragment represented a novel gene. Combination of Northern blot and virtual Northern and multiple tissues Northern blot, expression of the cDNA in multiple normal and carcinoma tissues were analyzed.
RESULTSOne of the important cDNA bands with poly(A) tail was cloned. This band was named W41. Sequence analysis showed that W41 consists of 533 bp. Basic local alignment search tool analysis revealed that W41 has low identity with any genes from GenBank. This sequence data was submitted to GenBank with accession No. AF 325202. Northern blot revealed that W41 presented higher expression in gastric cancer tissue than in normal tissue. Multiple tissue Northern blot revealed that W41 presented higher expression in multiple cancers than in normal tissues. Virtual Northern revealed that the cDNA presented higher expression in tumor series analysis of gene expression libraries than in normal.
CONCLUSIONA novel human gastric cancer-associated cDNA fragment was identified.
