Preliminary analysis on human telomerase reverse transcriptase gene expression in acute leukemia.
- Author:
Yan ZHANG
1
;
Shu-qiu LIU
;
Yi QU
;
Xi-hong LI
;
Feng-lan WANG
;
Wei DONG
;
Bai-lin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adolescent; Adult; Child; Child, Preschool; DNA-Binding Proteins; Female; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Humans; Infant; Leukemia; genetics; pathology; Male; Neoplasm Recurrence, Local; RNA, Messenger; genetics; metabolism; Remission Induction; Reverse Transcriptase Polymerase Chain Reaction; Telomerase; genetics
- From: Chinese Journal of Medical Genetics 2003;20(1):61-63
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between gene expression of human telomerase reverse transcriptase (hTERT) and its clinical characteristics in leukemia.
METHODSThe protocol of RT-PCR was used to detect the hTERTmRNA expressing levels in peripheral blood samples from leukemic patients under primary treatment(n=42), in complete remission(n=21), with recurrent leukemia (n=4); and from normal subjects (n=5), respectively.
RESULTSThe positive percentage of hTERTmRNA expression was 73.81% for the primary treatment cases, and 19.05% for the complete remission cases. All of the recurrent cases gave positive results. One of the normal controls presented low level of hTERTmRNA expression. The expressing level of hTERTmRNA in primary treatment cases was 0.64+/-0.21, in complete remission leukemia 0.31+/-0.16, in recurrent cases 0.84+/-0.09, and in normal controls 0.10.
CONCLUSIONThe activation of telomerase may be an essential factor in the development of leukemia and usually be the late event in its progression. As an indicator of leukemia cell, the detection of hTERT mRNA may be used in clinical analysis, disease monitoring and prognosis judgement.
