Expression of human anti-apoptotic gene survivin and its splice in normal human gastric tissue and gastric cancer.
- Author:
Xin-yan YANG
1
;
Meng-wei WANG
;
Gang-shi WANG
;
Wei-di YOU
Author Information
- Publication Type:Journal Article
- MeSH: Alternative Splicing; genetics; Base Sequence; Cloning, Molecular; DNA, Complementary; chemistry; genetics; Gene Expression Regulation, Neoplastic; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; genetics; Molecular Sequence Data; Neoplasm Proteins; Sequence Analysis, DNA; Stomach Neoplasms; genetics
- From: Chinese Journal of Medical Genetics 2003;20(1):75-76
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the expression of human anti-apoptotic gene survivin (SVV) in normal human gastric tissues and gastric cancer.
METHODSSVV cDNA clone was obtained from human gastric cancer tissues by virtue of RT-PCR, using Dig-marked cRNA probe in situ hybridization to analyze its expression in normal human gastric tissues and gastric cancer.
RESULTSTwo SVV cDNA clones, SVV-S4A and SVV-S1B were obtained. The sequence of the former is identical to that of the well-known SVV cDNA; however, in the sequence of the latter, the third exon was missed, i.e., there are only two exons in SVV-S1B. In situ hybridization showed that SVV-S4A is mainly expressed in gastric cancer tissues whereas SVV-S1B is mostly expressed in normal gastric tissues.
CONCLUSIONThere is difference between SVV-S4A and SVV-S1B in respect to their characteristics of expression in gastric cancer and normal gastric tissues.
