Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells.
- Author:
Gang LIU
1
;
Guangxiu LU
;
Junjiang FU
;
Shangfeng LIU
;
Xiaowei XING
;
Luyun LI
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Apoptosis; genetics; Base Sequence; Cloning, Molecular; DNA, Complementary; chemistry; genetics; Female; Gene Expression; Heat-Shock Proteins; Humans; Male; Mice; Molecular Sequence Data; Proteins; genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Spermatocytes; cytology; metabolism
- From: Chinese Journal of Medical Genetics 2003;20(2):107-110
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone a novel gene which is related to human testis spermatogenesis apoptosis.
METHODSTo rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed.
RESULTSThe present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method.
CONCLUSIONA novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.
