Detection of common chromosome abnormalities in myelodysplastic syndrome with a panel fluorescence in situ hybridization.
- Author:
Yongmei SHEN
1
;
Yongquan XUE
;
Jianyong LI
;
Jinlan PAN
;
Yafang WU
;
Suning CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Chromosome Aberrations; Chromosomes, Human, Pair 20; genetics; Chromosomes, Human, Pair 5; genetics; Chromosomes, Human, Pair 7; genetics; Chromosomes, Human, Pair 8; genetics; Female; Humans; In Situ Hybridization, Fluorescence; methods; Karyotyping; Male; Middle Aged; Myelodysplastic Syndromes; genetics
- From: Chinese Journal of Medical Genetics 2003;20(2):160-163
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the value of a panel fluorescence in situ hybridization (FISH) in the detection of common chromosome abnormalities in myelodysplastic syndrome (MDS).
METHODSTwenty cases of MDS patients, whose karyotypes were unknown by the FISH examiner beforehand, were analyzed with a panel FISH using YAC248F5 (5q31), YAC938G5 (7q32), CEP8 and YAC 912C3 (20q12) probes to detect the frequently occurring chromosome abnormalities (-5/5q, -/7q-, +8, 20q-) in MDS. Then the results were compared to those of conventional cytogenetics (CC).
RESULTSAmong 20 cases, 13 cases were found to carry common chromosome abnormalities by panel FISH (trisomy 8, five cases; -5/5q-, one case; 20q-, five cases; 5q- accompanying 20q-, one case; complex abnormalities, one case). However, on CC examination, only five cases were found to have common chromosomal abnormalities (20q-, four cases; 5q- accompanying 20q-, one case). In addition, trisomy 21, marker chromosome and complex abnormalities comprising -5, -7 and marker chromosomes were seen in one case each, the rest were normal.
CONCLUSIONPanel FISH is a useful tool of molecular cytogenetics in the detection of common chromosome abnormalities in MDS.
