Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay.
- Author:
Mo-bin WAN
1
;
Guan-shan ZHU
;
Rui-ying ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Biomarkers; Clinical Laboratory Techniques; DNA; Endonucleases; metabolism; Genetic Techniques; Genomic Imprinting; genetics; Humans; Pedigree; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Reverse Transcriptase Polymerase Chain Reaction; methods
- From: Chinese Journal of Medical Genetics 2003;20(3):225-227
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a novel approach for quick and high throughput verification of human gene imprinting.
METHODSBy use of a pair of dye-labeled probes, 5' nuclease assay was combined with reverse transcriptase-PCR(RT-PCR) to genotype a coding single nucleotide polymorphism (cSNP), rs705(C/T) of a known imprinted gene, small nuclear ribonucleotide protein N (SNRPN), on both genomic DNA and cDNA of human lymphoblast cell lines.
RESULTSAllele discrimination showed a clear monoallelic expression pattern of SNRPN, which was confirmed by RT-PCR based restriction fragment length polymorphisms. Pedigree analysis verified the paternal origin of expressed allele, which is in consistency with previous report.
CONCLUSIONCoding SNP is an ideal marker for detecting gene imprinting by 5' nuclease assay. This approach has also a potentiality to discover differential allele expression of non-imprinted genes in order to find gene cis-acting functional polymorphism.
