miR-200b suppresses glioma cell invasion by targeting PROM1.
- Author:
Biao PENG
1
;
Su HU
2
;
Mingjun QIN
2
;
Dongdong LUO
2
;
Xun ZHANG
2
;
Hailin ZHAO
2
Author Information
- Publication Type:Journal Article
- MeSH: 3' Untranslated Regions; AC133 Antigen; Antigens, CD; metabolism; Cell Line, Tumor; Down-Regulation; Genes, Reporter; Genes, Tumor Suppressor; Genetic Vectors; Glioblastoma; genetics; metabolism; Glycoproteins; metabolism; Humans; Luciferases; MicroRNAs; metabolism; Peptides; metabolism; RNA, Messenger; RNA, Small Interfering; Transfection
- From: Chinese Journal of Oncology 2015;37(1):25-28
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore whether miR-200b suppresses tumor cell invasion by targeting PROM1, thus to reveal the molecular mechanism that miR-200b functions as a tumor suppressor in glioma.
METHODSPROM1 3'UTR-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-200b on luciferase activity. Human glioblastoma U87 cells were transfected with miR-200b mimics, and next qRT-PCR and Western blotting were performed to detect the expressions of PROM1 mRNA and protein. The effect of PROM1 down-regulation on invasion was observed after PROM1 siRNA were transfected into U87 cells.
RESULTSThe miR-200b bound to the 3'-untranslated region (UTR) of PROM1 and inhibited the luciferase activity. Its luciferase activity was down-regulated by 57.0% (P < 0.01). PROM1 protein and mRNA expressions were significantly down-regulated when miR-200b was overexpressed in the U87 cells (P < 0.05). siRNA-mediated down-regulation of PROM1 suppressed the potential of cell invasion. The invasion ability of SKOV3 cells after transfection with siRNA-PROM1 was significantly lower than that in the negative control cells (P < 0.05).
CONCLUSIONmiR-200b may suppress cell invasion by targeting PROM1 in glioma.
