Effects of Simvastatin on osteoblast activity of human periodontal ligament cells.
- Author:
Fei HU
1
;
Xue-yang ZHANG
;
Chun-xian WANG
;
Lei ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; Cell Differentiation; Cells, Cultured; Humans; Osteoblasts; Osteogenesis; Osteopontin; Periodontal Ligament; Simvastatin
- From: West China Journal of Stomatology 2009;27(3):313-316
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Simvastatin on the osteoblast activity of human periodontal ligament (PDL) cells.
METHODSThe third passage human PDL were cultured in conditional mineralization medium with different concentrations of Simvastatin. The cells were divided into A group (0 mol/L), B group (1 x 10(-9) mol/L), C group (1 x 10(-8) mol/L), D group (1 x 10(-7) mol/L) and E group (1 x 10(-6) mol/L). Alkaline phosphatase (ALP) activity, osteopontin (OPN) and capability of mineralization were measured.
RESULTSDifferentiation osteoblast and mineralization of human PDL were improved in all treatment groups with different concentrations of Simvastatin (1 x 10(-9), 1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol/L). Compared with control group, statistically significant differences were found in 1 x 10(-8) mol/L, 1 x 10(-7) mol/L and 1 x 10(-6) mol/L groups (P<0.05). The maximum effect was observed at the concentration of 1 x 10(-7) mol/L.
CONCLUSIONOptimal concentration of Simvastatin can improve the osteoblastic activity of human PDL.
