Effects of oxymetazoline hydrochloride on ex vivo human nasal cilia movement measured with high-speed digital microscopy
10.3321/j.issn:1673-0860.2008.04.006
- VernacularTitle:盐酸羟甲唑啉对慢性鼻窦炎离体钩突黏膜纤毛细胞纤毛摆动频率的短期影响
- Author:
Xiao-Hong SONG
1
;
Luo ZHANG
;
De-Min HAN
;
Kui-Ji WANG
;
Hong WANG
;
Wei ZHANG
Author Information
1. 首都医科大学附属北京同仁医院
- Keywords:
Oxymetazoline;
Nasal mucosa;
Epithelial cells;
Cilia
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(4):268-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of oxymetazoline hydrochloride on ex vivo human nasal cilia movement.Methods Ciliary beat frequency (CBF) of cultured human nasal epithelial cells was measured by high-speed digital microscopy in HBSS and oxymetazoline hydrochloride of different concentrations in 20 minutes.Results CBF of cultured nasal epithelial cells in HBSS and 0.25 g/L oxymetazoline hydrochloride did not show significant changes in 20 minutes (F=0.098,P=1.00).However,in 0.50g/L and 1.00g/L oxymetazoline hydrochloride,CBF increased slightly in 3-4 minutes and reached the apex,then decreased gradually.At the end of observation,CBF showed no significant difference in 0.50g/L,(F=2.94,P=0.05) but there was a significant lower CBF in 1.00g/L.In the first 3 minutes,the CBF in 2.00g/L oxymetazoline hydrochloride was stable,and then slowed gradually.After 16 minutes,there was significant difference.In initial,the highest normalized CBF of each group showed no significant difference.However,the lowest normalized CBF of 1.00 and 2.00g/L oxymetazoline hydrochloride showed a significant difference with HBSS,0.25 and 0.50g/L oxymetazoline hydrochloride.Conclusions Oxymetazoline had a concentration-dependent inhibitory effect on cultured human nasal CBF from 0.25 to 2.00g/L.The inhibitory effect increasedwith the concentration going up.Oxymetazoline hydrochloride of 0.50g/L might be the optimal choice for clinical application.
