Expression of stathmin mRNA and protein in laryngeal squamous cell carcinoma and its clinical implication
10.3321/j.issn:1673-0860.2008.04.011
- VernacularTitle:Stathmin基因在喉鳞状细胞癌中的表达及临床意义
- Author:
Cai-Yun ZHANG
1
;
Zi-An XIAO
;
Yi-Ci ZENG
;
Xin-Ming YANG
;
Ding-Hua XIE
;
Kun XIA
;
Fu-You LIU
;
Bo-Yun HUANG
Author Information
1. 复旦大学附属上海市第五人民医院
- Keywords:
Stathmin;
Laryngeal neoplasms;
Carcinoma,squamous cell;
Immunohistochemistry
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(4):291-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of stathmin gene and its coding protein in laryngeal squamous cell carcinoma,and to explore the relationship between stathmin gene and the biological behaviors of laryngeal squamous cell carcinoma for understanding the tumorigenicity and development of laryngeal squamous cell carcinoma.Methods Laryngeal carcinoma tissues (studying group) in the tumors'center and laryngeal normal tissues (control group) parted from 1.0 cm of the safe borderline of the tumors were took from 38 patients with laryngeal squamous cell carcinoma while they were in operation.Semi-quantitative method of reverse transcriptase polymerase chain reaction (RT-PCR) was used to analyze the expression level of stathmin mRNA,and immunohistochemical staining (frozen section) was used to detect the expressions of stathmin protein,in laryngeal carcinoma tissues and laryngeal normal tissues of 38 cases,respectively.Results mRNA of stathmin gene was all positively expressed in laryngeal carcinoma tissues and in laryngeal normal tissues of 38 cases by RT-PCR.However,stathmin mRNA was obviously over-expressed in laryngeal carcinoma tissues than that in laryngeal normal tissues (t=9.655,P＜0.05).Immunohistochemical staining showed stathmin protein was positively expressed in laryngeal carcinoma tissues of 26 cases (26/38,68.4%),and mild-positively expressed in laryngeal normal tissues in 13 cases(13/38,34.2%).There was significant difference between the expression rate of stathmin protein inlaryngeal carcinoma tissues and in laryngeal normal tissues (x2=8.901,P＜0.05).Meanwhile,theexpression level of stathmin mRNA and the positive-expressed rate of stathmin protein in laryngeal carcinoma tissues of the advanced stage patients group(Ⅰ stage and Ⅳ stage) were significantly higher than these in laryngeal carcinoma tissues of Ⅰ and Ⅱ stage patients group (t=6.284,x2=5.810,P＜0.05),and they were also significantly higher in laryngeal carcinoma tissues of the patients group with cervical lymph node metastasis than in laryngeal carcinoma tissues of the patients group without cervical lymph node metastasis (t=9.350,x2=6.923,P＜0.05).Conclusions The expression levels of stathmin gene and protein were significantly higher in laryngeal squamous cell carcinoma than these in laryngeal normal tissues,the levels are also significantly higher in advanced stage patients group (Ⅲ stage and Ⅳ stage) than in the early stage patients group (Ⅰ and Ⅱ),and they are also related to the cervical lymph node metastasis of carcinoma.Stathmin gene may play an important role in the pathogenisis and development of laryngeal carcinoma and may be related to its prognosis.
