Overexpression of Hath1 induces production of hair cell-like cells in greater epithelial ridge cell cultures from postnatal rat cochlea
10.3321/j.issn:1673-0860.2008.05.010
- VernacularTitle:Hath1基因诱导新生大鼠大上皮嵴细胞形成毛细胞样细胞
- Author:
Yuan ZHANG
1
;
Yin-Yan HU
;
Suo-Qiang ZHAI
;
Wei GUO
Author Information
1. 首都医科大学附属北京同仁医院
- Keywords:
Cochlear;
Cell,cultured;
Transfection;
Hath1
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(5):360-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Hath1(human atonal homolog 1)overexpression on greater epithelial ridge (GER) cells from postnatal rat cochlea in vitro.Methods GER cells were isolated by using a combinatorial approach of enzymatic digestion and mechanical separation from P1 rat cochlear.The GER cell cultures were infected by adenovirus containing Hath1 and enhanced green fluorescent protein(ad-Hath1-EGFP),while transfecting EGFP(ad-EGFP) was as controls.Immunostaining were performed at different time points after adenovirus infection.Resuits Some of the infected GER cells became myosin Ⅶa-positive following ad-Hathl-EGFP infection.The earliest time point to see induction of hair cell differentiation(hair cell marker expression)by ad-Hath1 was 5 days post-infection.In contrast,infection of the GER sheet cultures with ad-EGFP control virus did not show any myosin Ⅶa-positive cells at 3-12 days post-infection in all cultures examined.Conclusions GER cells may potentially serve as hair cell progenitors and they are capable of difierentiating hair cell-like cells when forced to express Hath1.
