Effect of sodium salicylate on the auditory brain stem response threshold and expression of glutamic acid decarboxylase in spiral ganglion of juvenile and adult guinea pigs
10.3321/j.issn:1673-0860.2008.05.011
- VernacularTitle:水杨酸钠对幼年和成年豚鼠听性脑干反应阈值及螺旋神经节谷氨酸脱羧酶表达的影响
- Author:
Shi-Hua YIN
1
;
An-Zhou TANG
;
Song-Hua TAN
;
Ping CHEN
;
Li-Hong XIE
;
Yi REN
Author Information
1. 广西医科大学附属第一医院
- Keywords:
Sodium salicylate;
Evoked potentials,auditory,brain stem;
Glutamate deearboxylase;
Spiral ganglion;
Guinea pigs
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(5):364-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the differences of regulation of sodium salicylate on the auditory brain stem response(ABR)threshold and expression of glutamic acid decarboxylase(GAD)protein in spiral ganglion of juvenile and adult guinea pigs.Methods Fourty juvenile guinea pigs which were born just four days and fourty adult guinea pigs which were born thirty days were selected.They were divided four groups (group A;group B;group C;group D).ABR threshold was detected before administration.after administration for 15 days and after administration stopped for 30 days.The protein expression of GAD were measured after administration for 15 days and after administration stopped for 30 days by the method of immunohistochemistry. Results ABR threshold of juvenile sodium salicylate groups (group C) was increased remarkably than that of before administration and the control after administration for 15 days( P<0.001). ABR threshold of group C was returned to the level of that of before administration and after administration stopped for 30 days.ABR threshold of adult sodium salicylate groups (group D) was lncreased remarkably than that of before administration and the control after administration for 15 days ( P<0.001).ABR threshold of group D was kept the high level after administration stopped for 30 days.The protein expression of GAD of sodium salicylate groups(group C and D)was decreased than that of the control after administration ror 15 days.The protein expression of group C was more visible degression than that of group D(t=4.7,P<0.001).The protein expression of group C was retumed the level of beforeadministration afler administration stopped for 30 days,but the protein expression of group D was kept the high level.Conclusions The results suggest that sodium salicylate can regulate differently ABR threshold and expression of GAD protein in spiral ganglion of juvenile and adult guinea pigs.The effects of sodium salicylate on ABR threshold and expression of GAD protein in spiral ganglion of juvenile pigs are more noticeable than that of adult guinea pigs,but these changes are easier to return the normal than that of adult guinea pigs.
