Uptake and accumulation of gentamicin by mouse hair cells from the cochlear explants in vitro
10.3321/j.issn:1673-0860.2008.06.010
- VernacularTitle:体外培养小鼠耳蜗Corti器对庆大霉素的吸收
- Author:
Jian-Ping LIU
1
;
Chun-Fu DAI
;
Zheng-Min WANG
;
Fang-Lu CHI
;
Jie TIAN
;
Cui-Di DA
Author Information
1. 复旦大学附属眼耳鼻喉科医院
- Keywords:
Gentamicins;
Tissure distribution;
Organ of Corti;
Organ culture techniques
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(6):439-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the uptaking and accumulation of gentamicin by mouse hair cells in vitro.Methods Cochlear explants were prepared from the microdissected neonatM mouse cochlea. Cochlear explants were cultured with gentamicin-Texas-red conjunction (G1TrR) for difierent time.Laser confocal microscopy was used to observe the distribution of GTYR in the cochlear sensoty ceUs after labeling with phalloidin-alexa-488.ResulIs Soon after culture,there was diffuse red staining all tissue cells in the explants.At later time the hair cells were more staining tllan other cells in the explants.There was no obviously accumulation of GTTR in the supporting cells.The peak level of fluorescent density was reached at 24 hours culture.The GTTR was seen in the infracuticular zone of the hair cells.There was still accumulation of GTTR in the hair cells of the explants after 7 days culturing.Conclusions GTTR and Cochlea explants were useful methods to investigate the pharmacokinetics and mechanisms of gentamicin accumulation over time.
