Establishment and significance of the experimental minimal persistent inflammation models in allergic rhinitis guinea pigs
10.3321/j.issn:1673-0860.2008.06.011
- VernacularTitle:豚鼠实验性变应性鼻炎最轻持续炎性反应模型的建立
- Author:
Fei LEI
1
;
Dong-Dong ZHU
;
Hong KONG
;
Ya-Nan CUI
;
Yun-Peng JIANG
;
Zhen DONG
Author Information
1. 吉林大学中日联谊医院
- Keywords:
Models,animal;
Rhinitis,allergic,seasonal;
Eosinophils;
Intercellular adhesion molecule-1
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(6):443-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an animal model of minimal persistent inflammation(MPI)in allergic rhinitis guinea pigs and to investigate its significance.Methods Sixty male Hartley guinea pigs were divided into four groups:group A(positive control group),B(MPI model group),C(negative group) and D(bland group)respectively,with fifteen animals in each group.Guinea pigs from group A,B and C were sensitized intraperitoneally by injection of suspension of ovalbumin(OVA)and aluminum hydroxide in 0.9%physiological saline.Then,repeated local booster sensitization with different concentration of OVA suspension(1%and 0.01%)or physiological saline into the nasal cavity of those guinea pigs were performed.For group D,physiological saline was used only.Symptoms(sneezing)of guinea pigs after intercellular adhesion molecule 1(ICAM-1)in the nasal epithelial cells were also examined.Results When challenged with l%OVA.the sneezing number of guinea pigs in group B was increased markedly than that in group D(P<0.05).However,there was no difference between group B,A and C(P>0.05).When challenged with 0.01% OVA,the symptom of snee~ng almost disappeared in group B just like that in group D and there was no difference between the two groups(P>0.05). Besides,there was still more EOS infiltrated in the nasal mucosa of guinea pigs in group B than that in group D(P<0.05).There was no expressed in group B.Condusions MPI models have been established successfully through long term challenge with lower density of OVA in the sensitized guinea pigs.which will provide us witll a new method for further research in the mechanism and treatment of allergic rhinitis.
