Effect of Allopurinol on Methylmercuric Chloride in Cultured O1igodendrocytes.
10.11637/kjpa.1996.9.2.183
- Author:
Seung Taeck PARK
;
Byung Rim PARK
;
Yeun Tai CHUNG
- Publication Type:Original Article
- Keywords:
Methylmercury;
Cultured bovine oligodendrocyte;
Antioxidant;
Oxygen radicals
- MeSH:
Allopurinol*;
Antioxidants;
Cell Survival;
Neuroprotective Agents;
Oligodendroglia;
Reactive Oxygen Species
- From:Korean Journal of Physical Anthropology
1996;9(2):183-188
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To elucidate the neurotoxic mechanism of methylmercury on cultured bovine oligodendrocytes, neurotoxic effect was estimated by MTT assay after cultures were exposed to various concentrations of methylmercuric chloride (MMC). In addition, neuroprotective effect of antioxidant, allopurinol agonist MMC-induced neurotoxicity was examined on these cultures. Exposure of cultured bovine oligodendrocytes to MMC showed less than 50% of the cell viability 24 hours after treatment with 35µM of MMC. And also, allopurinol blocked the neurotoxicity induced by MMC on these cultures. These results suggest that oxygen radicals involve in MMC-mediated neurotoxicity, and also seletive antioxidants such as allopurinol are effective in blocking the neurotoxicity induced by MMC on cultured bovine oligodendrocytes.
