Effect of Allopurinol on Methylmercuric Chloride in Cultured O1igodendrocytes.
10.11637/kjpa.1996.9.2.189
- Author:
Seung Taeck PARK
;
Yeun Ja MUN
;
Jae Min OH
;
Jung Joong KIM
;
Min Kyu CHOI
;
Jae Han SHIM
;
Kye Taek LIM
;
Yeun Tai CHUNG
- Publication Type:Original Article
- Keywords:
Iron-chelator;
Neurotoxicity;
Oxygen radical;
ultured bovine oligodendrocyte
- MeSH:
Allopurinol*;
Cell Survival;
Hypoxanthine;
Neuroprotective Agents;
Oligodendroglia;
Reactive Oxygen Species;
Xanthine Oxidase
- From:Korean Journal of Physical Anthropology
1996;9(2):189-195
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to examine the neurotoxic mechanism of oxygen radicals on cultured bovine oligodendrocytes, cytoxic effect of oxygen radicals was examined when cultures were treated with various concentrations of xanthine oxidase (XO) and hypoxanthine (HX) in culture medium. In addition, the neuroprotective effect of iron-chelators against the neurotoxicity induced by oxygen radicals was evaluated by MTT assay. Cell viability was remarkably decreased in a time-dependent manner after exposure of cultured bovine oligodendrocytes to 20mU/ml XO and 0.1mM HX for 4 hours. In the neuroprotective effect of iron-chelators on oxidant-induced neurotoxicity, tetrakis (2-pyridylmethyl)ethylenediamine (TPEN) blocked the neurotoxicity induced by oxygen radicals, while DFX was not effective in blocking oxidant-induced neurotoxicity in these cultures. These results suggest that oxygen radicals are toxic in cultured bovine oligodendrocytes, and also selective iron-chelators such as TPEN are effective in blocking the neurotoxicity induced by oxygen radicals.
