Influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus following unilateral labyrinthectomy in guinea pigs.
- Author:
Jingcheng SHU
1
;
Shihua YIN
2
;
Yuan LIU
1
;
Xiuli RAO
1
;
Shunlian WEI
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Betahistine; metabolism; Ear, Inner; Guinea Pigs; Otologic Surgical Procedures; Receptors, Histamine H3; metabolism; Vestibular Nuclei; metabolism; Vestibule, Labyrinth; surgery
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2014;49(12):1027-1030
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL).
METHODSFifty-six healthy guinea pigs were randomly divided into three groups:the sham-operated group (group I), the UL group[group II, and UL+betahistine (BET) group (group III)], BET was intraperitoneally injection at 2.17 mg×kg(-1)×d(-1) for 7 days. The expression of histamine H3 receptor was analyzed by immunohistochemistry at 1 day, 3 days and 7 days after UL.
RESULTSH3 receptors were presented in the MVN and the expression of histamine H3 receptor were increased significantly in the ipsilateral MVN at 1 and 3 days after UL(P < 0.05), the change turned into the normal value at 7 days(P > 0.05). In the UL+BET group, the intensity of histamine H3 receptor was lower than that in the UL at 1 day and 3 days(4.25 ± 0.71, 3.50 ± 0.92 vs 5.75 ± 0.71, 5.50 ± 0.93, P < 0.05). However, the changes turned into the normal values at 3 and 7 days (P > 0.05).
CONCLUSIONSThe early stage of the vestibular compensation process may be associated with the change of H3 receptor expression in MVN. In the UL+BET group the histamine H3 receptor recovered quickly.
