Expression of hypoxia inducible factor-1α in chronic rhinosinusitis and the relationship with mucin secretion
10.3760/cma.j.issn.1673-0860.2015.02.010
- VernacularTitle:慢性鼻-鼻窦炎中缺氧诱导因子1α的表达及与黏蛋白分泌的关系
- Author:
Qing LUO
1
;
Hengtao ZHU
;
Kun FENG
;
Xiaoyan HUANG
;
Xueling JING
;
Jian ZHANG
Author Information
1. 330006,南昌大学第一附属医院耳鼻咽喉头颈外科
- Keywords:
Sinusitis;
Hypoxia-inducible factor 1,alpha sabunit;
Mucin 5AC;
Mucin 5B
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2015;50(2):138-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of hypoxia inducible factor-1α (HIF-1α) in chronic rhinosinusitis (CRS) and relationship with mucin MUC5AC,MUC5B secretion.Methods The expression of HIF-1 α,MUC5 AC and MUC5 B were measured by immunohistochemistry (IHC),Western Blot and reverse transcription polymerase chain reaction (RT-PCR) in CRS with or without nasal polyps (CRSwNP:twenty-three or CRSsNP:twenty)and fifteen normal sinus mucosa without CRS.The relationship between HIF-1α and MUC5AC,MUC5B were analyzed by SPSS 16.0 software.Results PAS and IHC showed that HIF-1α,MUC5AC,MUC5B,total mucins were expressed in nuclear of epithelium and gland cells,which were higher than normal control (t values in epithelium cells were 8.650,9.305,9.382,8.524,7.533,5.417,5.507,5.556,all P ＜ 0.05 ; t values in gland cells were 8.285,7.098,6.798,7.308,8.574,7.933,6.798,7.308,all P ＜ 0.05),but there was no significant difference between CRSwNP and CRSsNP (t values in epithelium cells were 0.734,0.415,0.572,0.248,all P ＞ 0.05 ; t values in gland cells were 0.331,0.662,0.249,0.644,P ＞ 0.05).Western Blot revealed that HIF-lα protein in CRSwNP and CRSsNP were higher than in normal contral (t values were 3.522,3.314,respectively,P ＜ 0.05).The relative expression levels of HIF-1α,MUC5AC,MUC5B mRNA in CRSwNP and CRSsNP were 1.35 ± 0.84,1.36 ± 0.94,0.81 ± 0.54,0.78 ± 0.46,1.13 ± 1.00,1.22 ± 1.02.But the relative expression levels of HIF-1 α,MUC5AC,MUC5 B mRNA in control subjects were 0.43 ± 0.34,0.42 ± 0.36,0.48 ± 0.42.There were significant differences between the groups of CRSwNP and control subiects(t values were 4.087,4.089,2.519,2.550,2.738,2.955,respectively,all P ＜ 0.05).There was a positive correlation among the expression of HIF-1 α and MUC5AC,5 B mRNA in CRSwNP (r values were 0.474,0.464,respectively,all P ＜0.05).There was a positive correlation among the expression of HIF-1α and MUC5AC,5B mRNA in CRSsNP (r values were 0.477,0.514,respectively,all P ＜ 0.05).Conclusions HIF-1 α,MUC5AC and MUC5B expression were upregulated in CRS with or without nasal polyps,indicating CRS is a disease of mucus hypersecretion and there is a close association between HIF-1α with hypersecretion of MUC5AC and MUC5B in mucus.
