NRP-1/shRNA silencing suppresses nasopharyngeal carcinoma cell growth in vitro and in vivo
10.3760/cma.j.issn.1673-0860.2015.04.010
- VernacularTitle:shRNA沉默NRP-1基因抑制鼻咽癌CNE-2Z细胞生长的体内外研究
- Author:
Jin SUN
1
;
Liang WANG
;
Weihua LOU
;
Hua CAO
;
Xiufen TIAN
;
Jianzhong SANG
Author Information
1. 450052,郑州大学第一附属医院咽喉头颈外科
- Keywords:
Neuropilin-1;
Nasopharyngeal neoplasms;
short hairpin RNA;
Gene therapy;
Xenograft tumors
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2015;50(4):312-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of RNA interference (RNAi) targeting NRP-1 gene on the growth and proliferation of nasopharyngeal carcinoma(NPC) CNE-2Z cells.Methods Short hairpin RNA(shRNA) plasmids targeting NRP-1 were designed and synthesized.These plasmids were respectively transfected into human NPC CNE-2Z cells and xenograft tumors in nude mice.The expressions of Fluorescein-labeled plasmids in NPC CNE-2Z cells and xenograft tumors were observed by fluorescence microscopy.Cell proliferation was detected by MTT assay.The inhibitory effects on target genes were evaluated with RT-PCR and Western blotting,respectively.The inhibitory effect of plasmids with the most effective sequence on xenograft tumors in nude mice was observed.Results CNE-2Z cell proliferation was significantly inhibited by NRP-1/shRNA silencing.RT-PCR showed NRP-1 mRNA expression was significantly decreased.Western blotting demonstrated the NRP-1/shRNA silencing can effectively inhibit the expression of target proteins in CNE-2Z cells.After six weeks,there were significant differences in the mean tumor volumes in nude mice between plasmid group and negative control group [(0.599 ± 0.002) vs (1.141 ± 0.013) cm3,P ＜ 0.05] or blank control group [(0.599 ± 0.002) vs (1.165 ± 0.308) cm3,P ＜0.05],and the inhibitory rate of tumor growth was 48.6％.Conclusion RNA interference targeting NRP-1 can remarkably inhibit the growth of CNE-2Z cells in vitro and in vivo.
