A mouse model for acute otitis media via transbullar injection
10.3760/cma.j.issn.1673-0860.2015.04.011
- VernacularTitle:听泡穿刺注射法构建C57BL/6小鼠急性中耳炎模型
- Author:
Yifei HUANG
1
;
Chunfang JIN
;
Yun XIANG
;
Lei WANG
;
Zimeng WANG
;
Wei WANG
;
Yujuan HE
Author Information
1. 400016,重庆医科大学检验医学院临床检验诊断学教育部重点实验室
- Keywords:
Disease models,animal;
Otitis media,suppurative;
Pneumococcal infections;
Mice,inbred C57BL
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2015;50(4):318-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a mouse model for acute otitis media (AOM) via transbullar injection method and evaluate its feasibility and practicability.Methods The middle ears (ME) of C57BL/6 mice were inoculated via transbullar injection method with 5 μl streptococcus pneumoniae (S.pn)19F suspension (1 × 107 CFU/ml),and the control group was inoculated equivalent phosphate buffered solution(PBS).Behavior changes were observed daily following inoculation.The ME tissues for histological examination and the middle ear lavage fluid (MELF) for total cells quantification,S.pn load determination and cytokines measurement were collected at 12 h,day 1,2,3,5,7 after inoculation,respectively.Results Within 24 hours after instillation,the density of S.pn and the level of acute inflammatory cytokines in ME cavity increased rapidly,some mucosal hyperplasia was evident and leukocytic infiltration (primarily neutrophils) began.The level of ME inflammatory response reached maximal at 2-3 days after inoculation,with extensive effusion,leukocytic infiltration and mucosal thickening.Meanwhile,the density of S.pn decreased gradually.Bacterial clearance was completed by day 5 with extensive resolution of ME inflammation,although mucosal hyperplasia did not resolute until day 7.Conclusion A mouse model for AOM is successfully established via transbullar injection method,laying foundation for future study of AOM.
