Preliminary study on aberrant expression of miRNAs related to the formation of the distinction between pediatric and adult types of brainstem gliomas.
- Author:
Xuan WANG
1
;
Hong-wei ZHANG
;
An-ling ZHANG
;
Lei HAN
;
Kun WANG
;
Pei-yu PU
;
Chang-hong SHEN
;
Chun-sheng KANG
;
Chun-jiang YU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Age Factors; Animals; Brain Stem; Brain Stem Neoplasms; metabolism; Child; Disease Models, Animal; Female; Gene Expression Profiling; Glioma; metabolism; Humans; In Situ Hybridization; MicroRNAs; metabolism; Oligonucleotide Array Sequence Analysis; Rats
- From: Chinese Journal of Surgery 2012;50(11):1015-1020
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the different expression of miRNA between pediatric and adult types of brainstem gliomas, and to provide the target miRNAs for explore the mechanism and miRNA interference of the malignant progression of pediatric BSG.
METHODSmiRNA expression profiles in orthotopic models which could simulate the BSG heterogeneity were examined by microarray and analyzed to obtain the aberrantly expressed miRNAs. The two types of human BSG tissue were utilized to verify the microarray data by qRT-PCR and in situ hybridization for the putative causative miRNAs.
RESULTSThere were 216 miRNAs detected in both the pediatric BSG group and the adult BSG group, 39 miRNAs to be differential expressed in the pediatric BSG group versus adult group, including 10 up-regulated and 29 down-regulated. qRT-PCR and in situ hybridization indicated good consistency with that of the microarray method.
CONCLUSIONSAberrantly expressed miRNA may serve as putative causative involvement of malignant progression of pediatric BSG, thereby might be potentially novel targets for therapy.
