The spontaneous YMDD mutation rate in chronic hepatitis B patients.
- Author:
Xiao-chun MIN
1
;
Xiao-hui MIAO
;
Shu-min ZHAO
;
Ke-kai ZHAO
;
De-gang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; DNA Primers; DNA, Viral; analysis; blood; genetics; Female; Genotype; Hepatitis B virus; genetics; Hepatitis B, Chronic; virology; Humans; Male; Middle Aged; Mutation; Polymerase Chain Reaction; methods; Sensitivity and Specificity; Viral Load; Young Adult
- From: Chinese Journal of Hepatology 2009;17(12):887-890
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the spontaneous YMDD mutation rate.
METHODSSerum samples collected from 196 untreated chronic HBV patients were detected by primer-specific real-time PCR.
RESULTSAmong 196 patients, spontaneous YMDD variants were detected in 21 subjects (20 YVDD mutants and 1 YIDD mutant). YMDD variants account for more than 50%, 25% to 50%, 9% to 25% of total virus load in 1, 5 and 15 patients, respectively. Gender, age, HBeAg status, serum viral load, the state of disease and duration of infection were not associated with spontaneous YMDD mutation. Genotype B had higher spontaneous YMDD rate than genotype C (20.00% vs 7.38%, x(2) = 6.28, P < 0.05).
CONCLUSIONSpontaneous YMDD variants exist in chronic hepatitis B patients, Genotype B is associated with higher spontaneous YMDD rate.
