Inhibitory effect of anluohuaxianwan on experimental hepatic fibrosis in rats.
- Author:
Xing-Hua TAN
1
;
Chang-Qing LI
;
Shang-Rong ZOU
;
Min XIE
;
Ai-Min ZHANG
;
Wen-Li LI
;
Xiao-Yue LI
;
Hui-Fen HUANG
;
Chun-Liang LEI
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Dimethylnitrosamine; Drug Combinations; Drugs, Chinese Herbal; pharmacology; therapeutic use; Hyaluronic Acid; blood; Hydroxyproline; metabolism; Immunohistochemistry; Liver; drug effects; metabolism; pathology; Liver Cirrhosis, Experimental; chemically induced; drug therapy; metabolism; pathology; Liver Function Tests; Male; Matrix Metalloproteinase 2; metabolism; Plants, Medicinal; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Hepatology 2010;18(1):9-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of anluohuaqianwan on experimental hepatic fibrosis induced by dimethyl nitrosamine (DMN) in rats.
METHODS36 male SD rats were randomly dividied into three groups: model group, normal group, anluohuaqianwan group. The rats in the three groups were treated with DMN daily for 4 weeks. The liver function was detected using auto biochemistry analyzer, the serum HA, LN, IV-C, PIIIP were detected by immunoradiometry, the histopathology was observed in the left liver lobe after HE staining, the expression of matrix metalloproteinase-2 (MMP-2) in liver tissue were detected by immunohistochemistry.
RESULTSThe serum levels of ALT, AST, ALP, TP, ALB and the contents of HA, LN, IV-C in model group were significantly increased compared to these in the normal group (P less than 0.01). The serum levels of ALT, AST and the contents of HA in anluohuaqianwan group were significantly lower than those in the model group (P less than 0.01). The liver MMP-2 in the model group was significantly increased compared to that in the normal group (P less than 0.05). The expression of MMP-2 in liver tissue of model group was lower than that in the anluohuaqianwan group (P less than 0.05).
CONCLUSIONAnluohuaqianwan can inhibit liver fibrosis in rats induced by DMN.
