Comparison of the Golgi proteome of hepatocellular carcinoma with that of the adjacent non-tumor tissues.
- Author:
Zhong XIAO
1
;
Yong-Fen YI
;
Ting-Ting HE
;
Yan-Qing LI
Author Information
- Publication Type:Journal Article
- MeSH: Annexin A5; analysis; metabolism; Carcinoma, Hepatocellular; metabolism; pathology; Electrophoresis, Gel, Two-Dimensional; methods; Golgi Apparatus; metabolism; Humans; Liver; metabolism; pathology; Liver Neoplasms; metabolism; pathology; Neoplasm Proteins; analysis; metabolism; Proteome; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Chinese Journal of Hepatology 2010;18(1):23-26
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the Golgi proteome of hepatocellular carcinoma (HCC) with that of the adjacent non-tumor tissues.
METHODSHepatocellular carcinoma and adjacent non-tumor tissues were obtained from HCC patients. The protein expression maps in Golgi were obtained by two-dimensional gel electrophoresis (2-DE), and the differentially expressed protein spots were analyzed by PD-Quest software. Peptide mass fingerprint (PMF) of differential protein spots was obtained with MALD-TOT-MS.
RESULTSAccording to 2-DE maps, the average numbers of protein spots were (1153+/-49) and (1086+/-37) in hepatocellular carcinoma and the adjacent non-tumor tissues. Compared to the adjacent non-tumor tissues, 27 proteins were upregulated, and 20 proteins were downregulated in HCC Golgi.
CONCLUSIONSThe Golgi proteome in HCC tissues is different from that in the adjacent non-tumor tissues, and the differential expression proteins are involved in energy metabolism, tumor metastasis, and cell cycle regulation.
