Effects of HBsAg pulsed dendritic vaccination on anti-HBs production in immunosuppressed rats after liver transplantation.
- Author:
Yue LI
1
;
Xian-Feng CHEN
;
Zuo-Jin LIU
;
Jian-Ping GONG
;
Sheng-Wei LI
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; pharmacology; Animals; Cytokines; blood; genetics; metabolism; Dendritic Cells; immunology; Disease Models, Animal; Hepatitis B; immunology; prevention & control; Hepatitis B Antibodies; blood; immunology; Hepatitis B Surface Antigens; immunology; Hepatitis B Vaccines; Immunosuppression; Immunosuppressive Agents; administration & dosage; Liver Transplantation; immunology; Male; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Inbred BN; Rats, Inbred Lew; Secondary Prevention; Spleen; immunology; metabolism
- From: Chinese Journal of Hepatology 2010;18(1):32-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of HBsAg pulsed dendritic vaccination on anti-HBs production in immunosuppressed rats after liver transplantation (LT).
METHODSBrown-Norway liver allografts were transplanted into Lewis recipients. The transplanted Lewis rats were injected with EK506 (2 mg/kg) and randomly divided into two groups: rats in HBsAg-DCs group (n = 15) were intraperitoneally injected with HBsAg pulsed DCs at 14 d and 28 d after LT, and rats in the HBsAg group (n = 15) were injected with HBsAg (200 mul) once a week for 12 weeks. Rats without any immunosuppressive treatment after LT served as controls (n = 5). IL-2 and IFN-gamma mRNA expression in spleen were analyzed by RT-PCR, serum IL-2, IFN-gamma and anti-HBs were detected by ELISA.
RESULTSHigh dose of FK506 resulted in the immunosuppressed in LT rats, as evident by low production of IL-2 and IFN-gamma, and without liver rejection compared to rats in the control group. HBsAg-DCs induced high titer of anti-HBs antibody, however, titer of anti-HBs were seldom detectable in the HBsAg group at 1, 2 and 3 mouth after vaccination.
CONCLUSIONThe capacity of HBsAg-DCs to induce anti-HBs in immunosuppressed rats suggested that DC vaccine may prevent HBV recurrence in liver transplanted patients.
