Identification of HBV genotype-specific tag sequences.
- Author:
Ying CAI
1
;
Xue-cheng LI
;
Xiao-mei WU
;
Ning WANG
;
Hong-wei CAO
;
Guo WEI
;
Ke-cen ZHAO
;
Kai ZHENG
;
Jiang ZHENG
;
Yan LI
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA Primers; Gene Library; Genes, Viral; Genotype; Hepatitis B virus; classification; genetics; Hepatitis B, Chronic; virology; Humans; Molecular Sequence Data; Polymerase Chain Reaction; methods; Protein Precursors; genetics; Sequence Analysis, DNA
- From: Chinese Journal of Hepatology 2010;18(2):101-104
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the HBV genotype-specific tag sequence.
METHODSThe large S region sequences from 930 HBV genomes were aligned to identify the genotype-specific tag sequences. PCR was used to check the genotyping effect of these tags.
RESULTSTwo tag sequences, sequence between 149-169 and sequence between 461-483, were identified in the large S region. Using primers specific to these tag sequences, the genotype of HBV can be specifically identified.
CONCLUSIONThese tag sequences can be used for HBV genotyping.
