Involvement of endoplasmic reticulum stress in development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats.
- Author:
Yong-ping MU
1
;
Norifumi KWADA
;
Tomohiro OGAWA
;
Xiu-hong XI
;
Xiao-rong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Caspases; genetics; metabolism; Cell Proliferation; Choline; administration & dosage; metabolism; Choline Deficiency; Diet; Disease Models, Animal; Endoplasmic Reticulum; physiology; Fatty Liver; complications; Liver; metabolism; pathology; Liver Cirrhosis; diet therapy; etiology; physiopathology; Male; Methionine; deficiency; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Wistar
- From: Chinese Journal of Hepatology 2010;18(2):124-130
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study role of endoplasmic reticulum stress in the development of fatty liver fibrosis induced by methionine-choline-deficient diet in rats.
METHODSNon-alcoholic steatohepatitis was induced by 10 weeks- methionine-choline-deficient diet (MCDD), Markers of endoplasmic reticulum stress were determined by immunoblotting and real-time PCR.
RESULTSThe number of apoptotic hepatocytes, The expression levels of endoplasmic reticulum stress markers were increased significantly in MCDD group compared to control group (probability value less than 0.05 or probability value less than 0.01), while ratio of hepatocyte proliferation/apoptosis was decreased in MCDD group (probability value less than 0.01). The number of hepatocytes apoptosis, and the expression levels of endoplasmic reticulum stress markers were decreased significantly 2 weeks after the feeding with normal diet in MCDD group (probability value less than 0.05 or probability value less than 0.01).
CONCLUSIONMCDD induces endoplasmic reticulum stress and fibrosis in rats.
