Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines.
- Author:
Rong-kuan LI
1
;
Jian-wu TANG
;
Jun ZHANG
;
Shao-qing WANG
;
Mei WANG
;
Bo WANG
;
Yu-hong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Hepatocellular; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; Chloride Channels; antagonists & inhibitors; genetics; metabolism; Gene Expression Regulation, Neoplastic; Genetic Vectors; Liver Neoplasms; metabolism; pathology; Mice; Neoplasm Invasiveness; Plasmids; genetics; RNA Interference; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Reverse Transcriptase Polymerase Chain Reaction; Transfection
- From: Chinese Journal of Hepatology 2010;18(2):131-135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.
METHODSThe mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.
RESULTSThe pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.
CONCLUSIONCLIC1 is essential for the proliferation and invasion of Hca-F cells.
