Characteristics of CDR3 of TCR beta on CD8+ T cells in chronic hepatitis B patients.
- Author:
Yong-Yin LI
1
;
Shi-Wu MA
;
Guang-Wen ZHANG
;
Xuan HUANG
;
Xiao-Xiong HU
;
Ling YANG
;
Ke ZHANG
;
Jin-Lin HOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; CD8-Positive T-Lymphocytes; immunology; Complementarity Determining Regions; genetics; Genes, T-Cell Receptor beta; Hepatitis B, Chronic; blood; immunology; Humans; Male; Receptors, Antigen, T-Cell; immunology; Young Adult
- From: Chinese Journal of Hepatology 2010;18(3):184-188
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the characteristics of CDR3 of TCRbeta on CD8+ T cells in chronic hepatitis B patients.
METHODSEight patients with chronic hepatitis B (ALT more than 2 ULN) were enrolled in this study. CD8+ T cells were isolated from peripheral blood. RT-PCR was proformed to amplify the CDR3 of TCRbeta, and the PCR products were sequenced and analyzed.
RESULTSThe chronic hepatitis B patients showed obvious clonal expansion of T cell, and three perturbation patterns of T cell expansion were showed in the CDR3 of TCRbeta, including monoclonicity, oligoclonicity and skewed peak patterns. The number of perturbation families of CD8+ subpopulation was significantly higher than that of CD8- subpopulation (10.6+/-4.7 vs. 4.1+/-3.1, t = 6.619, P less than 0.01). In 3 out of 8 patients, the number of perturbation families of CD8+ subpopulation was also higher than that of PBMCs without depleting CD8+ subpopulation.
CONCLUSIONSThe characteristics of CDR3 of TCRbeta may help to understand the inflammatory response in CHB patients.
