Effect of hydroxycamptothecin (HCPT) on proliferation and apoptosis of rat hepatic stellate cells.
- Author:
Yi ZHANG
1
;
Qun DENG
;
Guo-Xin HU
;
Keng YUAN
;
Fang YUAN
;
Yan-Qin HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Camptothecin; analogs & derivatives; pharmacology; Cell Line; Cell Proliferation; drug effects; Hepatic Stellate Cells; drug effects; Rats
- From: Chinese Journal of Hepatology 2010;18(3):199-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of hydroxycamptothecin (HCPT) on proliferation and apoptosis of rat hepatic stellate cells (HSC).
METHODSRat HSC line (HSC-T6) and rat hepatocyte line (BRL-3A) were treated with different concentrations of HCPT (0, 0.008, 0.016, 0.031, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32 mg/L respectively) for 24 h. Cell proliferation was assessed by MTT colorimetric assay, apoptosis was detected with PI staging followed by flow cytometry, and by DNA ladder assay. The morphological change of apoptosis was observed under transmission electron microscopy (TEM).
RESULTSMTT assay indicated that HCPT significantly inhibited the proliferation of HSC-T6 and BRL-3A in a dose-dependent manner. 24 h after the treatment with different concentrations of HCPT (0.25, 0.5, 1 mg/L), the apoptosis rate (13.46%+/-2.42%, 26.25%+/-5.65%, 47.05%+/-8.76%, respectively) in HSC-T6 was significantly higher than that in control cells (4.89%+/-1.80%, F = 34.24, P less than 0.01). 24 h after 0.5 mg/L HCPT treatment, cell shrinkage, nucleoli disappearance, chromatin condensation were found under TEM, and DNA ladder was demonstrated by agarose gel electrophoresis.
CONCLUSIONHCPT could significantly inhibit proliferation and induce apoptosis of HSC-T6 in a dose-dependent manner.
