Phenylhexyl isothiocyanate (PHI) regulates histone methylation and acetylation and induces apoptosis in SMMC-7721 cells.
- Author:
Yi-Qun HUANG
1
;
Xu-Dong MA
;
Ya-Dong LAI
;
Xiao-Zhong WANG
;
Jen-Wei CHIAO
;
De-Long LIU
Author Information
- Publication Type:Journal Article
- MeSH: Acetylation; Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Histones; metabolism; Humans; Isothiocyanates; pharmacology; Methylation
- From: Chinese Journal of Hepatology 2010;18(3):209-212
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of PHI on histone acetylation and methylation in hepatocellular carcinoma line SMMC-7721 cells.
METHODSApoptosis was measured by TUNNEL assay. Histone methylation and acetylation were detected by Western blot.
RESULTSPHI inhibited cells growth and induced apoptosis. PHI treatment resulted in increased acetylation of histone H3 and H4 , elevated level of histone H3 lysine 4 methylation, and decreased level of histone H3 lysine 9 methylation.
CONCLUSIONSPHI can modulate both histone acetylation and methylation, which could remodel chromatin structure. PHI may be a novel anticancer drug.
