Overexpression of adiponectin prevents hepatocyte steatosis.
- Author:
Jing ZHOU
1
;
Wen SUN
;
Zhou ZHOU
;
Ying WU
;
Ting-Xiu XIANG
;
Zheng JIANG
;
Xiao-Hong TAO
;
Ai-Long HUANG
;
Pi-Long WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adiponectin; genetics; Cell Line; Fatty Acids, Nonesterified; analysis; Fatty Liver; metabolism; Genetic Vectors; Glycerol; analysis; Hepatocytes; cytology; metabolism; Humans; Plasmids; Recombinant Fusion Proteins; genetics; Transfection; Triglycerides; analysis
- From: Chinese Journal of Hepatology 2010;18(3):213-216
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of adiponectin on hepatocyte steatosis.
METHODSL02 cells were transfected with pEGFP-N1-AdipoQ, a plasmid encoding pEGFP-adiponectin fusion protein, or pEGFP-N1. Lipid droplets in the hepatocytes were observed by oil red staining at 72 h. The contents of TG, FFA and glycerol in hepatocytes were measured.
RESULTSCompared to cells transfected with pEGFP-N1-AdipoQ plasmid, much more lipid droplets were observed in cells transfected with pEGFP-N1 plasmid. TG, FFA and glycerol contents in L02 cells and L02/pEGFP-N1 cells were significantly higher than those in L02/pEGFP-N1-AdipoQ cells.
CONCLUSIONSOverexpression of adiponectin prevent hepatocyte steatosis.
